Rec'd recombination
نویسندگان
چکیده
منابع مشابه
Exonuclease requirements for recombination of λ phage in recD mutants of Escherichia coli
Recombination of λ red gam phage in recD mutants is unaffected by inactivation of RecJ exonuclease. Since nucleases play redundant roles in E. coli, we inactivated several exonucleases in a recD mutant and discovered that 5’-3’ exonuclease activity of RecJ and exonuclease VII is essential for λ recombination, whereas exonucleases of 3’-5’ polarity are dispensable. The implications of the presen...
متن کاملThe genetic dependence of recombination in recD mutants of Escherichia coli.
RecBCD enzyme has multiple activities including helicase, exonuclease and endonuclease activities. Mutations in the genes recB or recC, encoding two subunits of the enzyme, reduce the frequency of many types of recombinational events. Mutations in recD, encoding the third subunit, do not reduce recombination even though most of the activities of the RecBCD enzyme are severely reduced. In this s...
متن کاملFunctions of multiple exonucleases are essential for cell viability, DNA repair and homologous recombination in recD mutants of Escherichia coli.
Heterotrimeric RecBCD enzyme unwinds and resects a DNA duplex containing blunt double-stranded ends and directs loading of the strand-exchange protein RecA onto the unwound 3'-ending strand, thereby initiating the majority of recombination in wild-type Escherichia coli. When the enzyme lacks its RecD subunit, the resulting RecBC enzyme, active in recD mutants, is recombination proficient althou...
متن کاملFocus 33 Integrating RECD fitting process
sound pressure level (SPL) directly; however, in certain populations (such as infants and children) the probe tube may not be tolerated for extended periods of time. An alternative is to derive (sometimes called “predict”) the real-ear SPL by adding an individually measured acoustic transfer function to both, the audiometric and electroacoustic data. The acoustic transfer function in question i...
متن کاملChromosomal transformation of Escherichia coli recD strains with linearized plasmids.
Wild-type Escherichia coli are resistant to genetic transformation by purified linear DNA, probably in part because of exonuclease activity. We demonstrate that E. coli containing a recD mutation could be easily transformed by linearized plasmids containing a selectable marker. The marker was transferred to the chromosome by homologous recombination, whereas plasmid markers not in the region of...
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ژورنال
عنوان ژورنال: Journal of Cell Biology
سال: 2005
ISSN: 1540-8140,0021-9525
DOI: 10.1083/jcb1701rr3